Coding

Part:BBa_K382041:Design

Designed by: Mara C. Inniss   Group: iGEM10_Harvard   (2010-10-26)


Arabidopsis optimized Brazzein+2xYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830
    Illegal BsaI.rc site found at 1580


Design Notes

Conforms to Silver standard 21


Source

Brazzein was synthesized. YFP tag was added by cloning

References